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Intracellular Ca2+-Release Channels as Cellular Targets for Immunophilins
A Molecular, Functional, and Structural Analysis

By Geert Bultynck
December 2001
Leuven University Press
ISBN: 90-5867-189-5
130 pages, illustrated
$60.00 paper original

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Contents:
Introduction. Cellular Ca-2+ signaling toolkit. The ryanodine receptor. The inositol 1,4,5-triphosphate receptor. Immunophilins. Aims. Experimental methods. Heterologous expression. Cell culture. Transfections. Preparation of microsomes. Recombinant proteins. Protein quantification and analysis. Immunoprecipitation of RyR1 and IP3R1. GST-FKBP12 and GST-FKBP12.6 affinity chromatography. Controlled proteolysis of RyR1, RyR3, and IP3R1. Functional assays. Secondary structure prediction of primary amino acid sequences of peptides. Results and discussions. Conclusions and perspectives. Summary. References.

Ph.D. thesis.

Biochemistry
Acta Biomedica Lovaniensia, No. 249